林麝IL-1β基因的克隆、表达及生物学活性检测

doi:10.11843/j.issn.0366-6964.2013.11.006

Abstract

Abstract:

The aim of this study was to clone and expression IL-1β from Moschus berezovskii for further study on the role of IL-1β in infectious diseases. Moschus berezovskii IL-1β (MBIL-1β) was cloned from peripheral blood mononuclear cells using RT-PCR. The sequence encoding the IL-1β mature peptide was ligated with pET32a(+) vector and expressed in Escherichia coli. The fusion protein was purified by His-Bind Column. Results showed that the length of MBIL-1β was 801 bp, encoding 266 amino acids. Sequence analysis showed that MBIL-1β had a high homology with other ruminants species. The M. berezovskii recombinant IL-1β (MB-rIL-1β) protein was expressed with IPTG induction and mainly in soluble form, the molecular weight was 35 ku. After purified, there was an aimed protein obtained. MTT essay confirmed that the MB-rIL-1β protein could enhance mouse fibroblast cell (L929) proliferation obviously and had biological activities. The result provide a feasible and convenient approach to produce soluble protein MB-rIL-1β with biological activity.

CLC Number:

ZOU Dan-dan, YANG Dong, JIANG Li-chun, ZOU Fang-dong. Molecular Cloning, Expression and Biological Activities Detection of Interleukin-1β (IL-1β) Gene from Moschus berezovskii[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(11): 1734-1738.

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Molecular Cloning, Expression and Biological Activities Detection of Interleukin-1β (IL-1β) Gene from Moschus berezovskii

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